Human Syndecan-1 Protein, Accession: AAH08765

Overview

General Description

Recombinant Human Syndecan-1 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln23-Gly254.

Biotinylated

Specifications

Target

Syndecan-1

Synonyms

Syndecan-1; SYND1; CD138; SDC1; SDC; SDC1

Accession

AAH08765

Species

Human

Exact Sequence

Gln23-Gly254

Molecular Weight

The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Syndecan-1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 19.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

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