Human SIRP alpha/CD172a Protein, Accession: P78324

/in /by
  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
  • Links

    • Overview

    General Description

    Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Glu31-Arg370.

    Biotinylated

    Specifications

    Target

    SIRP alpha/CD172a

    Synonyms

    CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3

    Accession

    P78324

    Species

    Human

    Exact Sequence

    Glu31-Arg370

    Molecular Weight

    The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.

    Activity

    Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.

    Product Tag

    C-hFc

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-SIRP-alpha/CD172a-Protein-accession-P78324/.