Human RGMa Protein, Accession: Q96B86

Overview

General Description

Recombinant Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Cys48-Gly422.

Biotinylated

Specifications

Target

RGMa

Synonyms

RGMA; RGM

Accession

Q96B86

Species

Human

Exact Sequence

Cys48-Gly422

Molecular Weight

The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 59.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

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This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-RGMa-Protein-accession-Q96B86/.