Human IL-15RA&IL-15 Protein, Accession: Q13261
- Overview
- Specifications
- Shipping, Reconstitution & Storage
- Links
Overview
General Description
Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).
Biotinylated
Specifications
Target
IL-15RA&IL-15
Synonyms
IL-15 R alpha；CD215；IL15RA；IL-15RA；IL-15R-alpha1；Interleukin-15；IL-15；IL15
Accession
Species
Human
Exact Sequence
Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Molecular Weight
The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 72.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Declaration
This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.
This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-IL-15RA&IL-15-Protein-accession-Q13261/.
