Human GDF15 Protein, Accession: Q99988

  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
  • Links
  • Overview

    General Description

    Recombinant Human GDF15 Protein is expressed from Expi293 with mFc (IgG2a) tag at the N-terminal.It contains Ala197-Ile308.

    Biotinylated

    Specifications

    Target

    GDF15

    Synonyms

    Accession

    Q99988

    Species

    Human

    Exact Sequence

    Ala197-Ile308

    Molecular Weight

    The protein has a predicted MW of 37.46 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

    Activity

    Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15, mouse IgG2a Fc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.

    Product Tag

    N-mFc

    Express System

    Expi293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per μg by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-GDF15-Protein-accession-Q99988/.