Human EphB3 Protein, Accession: P54753

Overview

General Description

Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gly34-Leu559.

Biotinylated

Specifications

Target

EphB3

Synonyms

EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1

Accession

P54753

Species

Human

Exact Sequence

Gly34-Leu559

Molecular Weight

The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Activity

Product Tag

C-His

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

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