Human Complement C5 Protein, Accession: P01031

  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
  • Links
  • Overview

    General Description

    Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln19-Cys1676.

    Biotinylated

    Specifications

    Target

    Complement C5

    Synonyms

    Accession

    P01031

    Species

    Human

    Exact Sequence

    Gln19-Cys1676

    Molecular Weight

    Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result.

    Activity

    Immobilized Human Complement C5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.

    Product Tag

    C-His

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-Complement-C5-Protein-accession-P01031/.