Human CA9/Carbonic Anhydrase IX Protein, Accession: Q16790

Overview

General Description

Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln38-Asp414.

Biotinylated

Specifications

Target

CA9/Carbonic Anhydrase IX

Synonyms

CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC

Accession

Q16790

Species

Human

Exact Sequence

Gln38-Asp414

Molecular Weight

The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CA9, His Tag at 0.9ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human-CA9/Carbonic-Anhydrase-IX-Protein-accession-Q16790/.