FPOX/Fructosyl-peptide Oxidase CAS UENA-0245

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Identification

CAS Number

UENA-0245

Name

FPOX/Fructosyl-peptide Oxidase

EC number

1.5.3 (Recombinant from microorganism)

Molecular Weight

60 kDa (SDS-PAGE)

Properties

Appearance

Yellow amorphous powder, lyophilized

Isoelectric Point

6.4

Michaelis constants

4.0×10-3 M (fructosyl-Val-His)

Inhibitors

Hg2+, Pb2+

Optimum pH

6.5-7.5

Fig. 1 Optimum pH
Fig. 1 Optimum pH

Optimum temperature

37 ℃

Fig. 2 Optimum temperature
Fig. 2 Optimum temperature

pH stability

pH 6.5-9.5 (25 ℃, 16 h)

Fig. 3 pH Stability
Fig. 3 pH Stability

Thermal stability

Below 40 ℃ (pH 8.0, 30min)

Fig. 4 Thermal stability
Fig. 4 Thermal stability

Storage stability

At least one year at -20 ℃

Fig.5 Storage stability (-20 ℃)
Fig.5 Storage stability (-20 ℃)

Stabilizers

Glycerol, trehalose

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our FPOX/Fructosyl-peptide Oxidase CAS UENA-0245

Protein purity

≥90% (from SDS-PAGE)

Activity

≥4 U/mg solid

Catalase

≤0.01%

ATPase

≤0.005%

Glucose oxidase

≤0.03%

Cholesterol oxidase

≤0.003%

Unit definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of H2O2 per min under the conditions described below.

Shelf Life

2 years

Storage

Under room temperature away from light

Known Application

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).