Fluorescein potassium salt CAS 6417-85-2

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• Properties
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Identification

CAS Number

6417-85-2

Name

Fluorescein potassium salt

Synonyms

dipotassium;3-oxospiro[2-benzofuran-1,9′-xanthene]-3′,6′-diolate

SMILES

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)[O-])OC5=C3C=CC(=C5)[O-].[K+].[K+]

StdInChI

InChI=1S/C20H12O5.2K/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

StdInChIKey

DZSPMLRDMGJVAT-UHFFFAOYSA-L

Molecular Formula

C20H13KO5

Molecular Weight

408.5

EINECS

229-143-9

Properties

Appearance

Pale yellow solid

Solubility

Soluble in water, DMSO, DMF, poorly soluble in dichloromethane, petroleum ether

Solubility (solvent: water, temperature: 25℃)

35 mg/mL

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Fluorescein potassium salt CAS 6417-85-2

Identification Methods

HNMR, HPLC

Purity

96% min

Maximum absorption wavelength

328 nm

Maximum emission wavelength

531 nm

Shelf Life

2 years

Storage

Under room temperature away from light

Features

1. The fluorescence is strongest under alkaline conditions, showing green fluorescence;
2. Usually used in aqueous solution (such as 40% aqueous solution);
3. Relatively high safety, environmentally friendly when used in small amounts.

Known Application

1. Dyes and tracers
   Fluorescent tracer:
   Commonly used to detect liquid flow path, leakage location, groundwater flow direction, water pipe leakage, etc.
   Water pollution tracking:
   In the field of environmental protection, it is used to track the diffusion path of pollutants in water bodies.
2. Biomedical and analytical applications
   Cell staining agent:
   Used as a fluorescent dye when observing cells, tissues or biological structures under a microscope.
   Ophthalmological examination:
   Used for corneal staining test to check corneal damage or foreign matter (such as used with a blue light slit lamp).
   Capillary electrophoresis, electrophoresis analysis:
   As a fluorescent labeling reagent to improve detection sensitivity.
3. Industrial detection
   Pipeline detection:
   Adding this dye to petrochemical, refrigeration, air conditioning and other systems, and irradiating with ultraviolet light can locate the leak point.
   CIP validation:
   In the pharmaceutical and food industries, it is used to confirm whether the cleaning process is thorough.

General View of Documents

Links

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).