D-xylose dehydrogenase CAS 62931-20-8

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Identification

CAS Number

62931-20-8

Name

D-xylose dehydrogenase

Synonyms

CvXylB

Molecular weight

27kDa

Properties

Appearance

Colorless clear to very slightly hazy liquid

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our D-xylose dehydrogenase CAS 62931-20-8

Product Description

CvXylB (Xylose Dehydrogenase), EC: 1.1.1.175, is derived from Caulobacter vibrioides and participates in the degradation of D-xylose—a primary constituent of hemicelluloses such as xylan. It catalyzes the initial reaction of the xylose utilization pathway by oxidizing D-xylose into D-xylono-1,4-lactone. It exhibits some activity toward L-arabinose but demonstrates negligible activity toward D-arabinose, L-xylose, D-ribose, D-galactose, D-glucose, or D-glucose-6-phosphate.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of D-xylose-1,5-lactone from D-xylose at 37°C and pH 9.0.

Identification Methods

HNMR, HPLC

Purity

＞90%

Enzyme activity

≥1U/mg

Buffer

20mMTris-HCl, pH7.5, 200mM NaCl, 10%glycerol

Suggested Procedure

  1. Add the substrate to the reaction system; optionally, add a buffer salt (e.g., Tris-HCl) to adjust the pH to 9.0, followed by the addition of an appropriate amount of CvXylB (based on reaction requirements).
  2. After mixing, incubate the sample at 37°C for 1–2 hours.
    [Note] When scaling up the reaction system, the incubation time or enzyme dosage should be adjusted according to actual conditions; low-speed shaking may be used to facilitate mixing.

Storage

Transport with ice packs or dry ice; store at -20°C;

Shelf Life

18 months.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).