Cynomolgus IL-33 Protein, Accession: A0A2K5W3I1

Overview

General Description

Recombinant Cynomolgus IL-33 Protein is expressed from HEK293 with His tag at the N-terminal.It contains His109-Ile270.

Biotinylated

Specifications

Target

IL-33

Synonyms

C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2

Accession

A0A2K5W3I1

Species

Cynomolgus

Exact Sequence

His109-Ile270

Molecular Weight

The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 46.85 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

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