Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein, Accession: QHO60594

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  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
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    • Overview

    General Description

    Recombinant Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H).

    Biotinylated

    Yes

    Specifications

    Target

    Spike S1 (Omicron B.1.1.529)

    Synonyms

    S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

    Accession

    QHO60594

    Species

    SARS-COV-2

    Exact Sequence

    Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)

    Molecular Weight

    The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

    Activity

    Immobilized Biotinylated Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA. See testing image for detail.

    Product Tag

    C-His-Avi

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% saccharose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-SARS-COV-2-Spike-S1-(Omicron-B.1.1.529)-Protein-accession-QHO60594/.