Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein, Accession: O00220

  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
  • Links
  • Overview

    General Description

    Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Pro34-Asn239.

    Biotinylated

    Yes

    Specifications

    Target

    TRAIL R1/DR4/TNFRSF10A

    Synonyms

    DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365

    Accession

    O00220

    Species

    Human

    Exact Sequence

    Pro34-Asn239

    Molecular Weight

    The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

    Activity

    Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test). The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.

    Product Tag

    C-His-Avi

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-Human-TRAIL-R1/DR4/TNFRSF10A-Protein-accession-O00220/.