Biotinylated Human Siglec-3/CD33 Protein, Accession: P20138
Overview
General Description
Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Asp18-His259.
Biotinylated
Yes
Specifications
Target
Siglec-3/CD33
Synonyms
Accession
Species
Human
Exact Sequence
Asp18-His259
Molecular Weight
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Liquid
Shipping Conditions
Shipped with blue ice.
Formulation
Supplied as 0.22um filtered solution in 20mM PB, 500mM NaCl (pH 8.0).
Reconstitution
Stability & Storage
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.
Declaration
This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.
This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-Human-Siglec-3/CD33-Protein-accession-P20138/.
