Biotinylated Human P53 R175H (HLA-A*02:01) Protein, Accession: A0A140T913

Overview

General Description

Recombinant Biotinylated Human P53 R175H (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide.

Biotinylated

Yes

Specifications

Target

P53 R175H (HLA-A*02:01)

Synonyms

MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53

Accession

A0A140T913

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide

Molecular Weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-P53 R175H (HLA-A*02:01) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 1.6ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Declaration

This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-Human-P53-R175H-(HLA-A*02:01)-Protein-accession-A0A140T913/.