- Shipping, Reconstitution & Storage
Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Ile20-Ser134.
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 90.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.
This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-Human-IL-5-Protein-accession-P05113/.