- Shipping, Reconstitution & Storage
Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Leu26-Pro247.
CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Immobilized Human CTLA-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-2, His Tag with the EC50 of 3.8ug/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.
This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Biotinylated-Human-B7-2/CD86-Protein-accession-P42081/.