α-Mannosidase CAS 9025-42-7

Identification

CAS Number

9025-42-7

EC Number

3.2.1.24

Name

α-Mannosidase CAS 9025-42-7

Synonyms

ALPHA-MANNOSIDASE;
ALPHA-D-MANNOSIDEMANNOHYDROLASE;
ALPHA1,6-MANNOSIDASE;
ALPHA1-2,3-MANNOSIDASE;
EC3.2.1.24;
a1,2Mannoisdase,Trichodermareesei,Recomb.,Saccharomyces;
a1,6-Mannosidase,Xanthomonassp.,Recomb.,E.coli;
a1-2,3-Mannosidase,Recomb.,E.coli

Properties

Physical form

Suspension in 50 mM PB buffer, pH 6.5

Molecular weight

220 kDa (SDS-PAGE)

Michaelis constant

5.2 ×10-4mol/L (p-nitrophenyl α-D-mannopyranoside)

Optimum pH

6.0

Optimum temperature

70 ℃

pH Stability

5~7 (25 ℃, 24 hrs )

Thermal stability

60 ℃ (pH 6.0, 24hrs)

Inhibitors

EDTA, swainsonine

Specifications and Other Information of Our α-Mannosidase CAS 9025-42-7

Unit definition

One unit of activity is defined as the amount of enzyme that released 1 μmol of p-nitrophenol per min at pH 6.0 and 60°C using p-nitrophenyl α-D-mannopyranoside as a substrate.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides and cleaves off α-1,2-, α-1,3-, α-1,4-, and α-1,6-linked mannose residues.

Specific activity

≥30 U/mg protein

Shelf Life

2 years at 4°C

Storage

Store at 2-8°C for long time, Sealed and away from light

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).